A0A1L9WN31 · ACUC_ASPA1

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of aculins (PubMed:26374386).
The pathway begins with the synthesis of 6-methylsalicylic acid by the polyketide synthase (PKS) acuA via condensation of acetate and malonate units (PubMed:26374386).
The 6-methylsalicylic acid decarboxylase acuB then catalyzes the decarboxylation of 6-methylsalicylic acid to yield m-cresol (also known as 3-methylphenol) (Probable). These first reactions occur in the cytosol (By similarity).
The intermediate m-cresol is then transported into the endoplasmic reticulum where the cytochrome P450 monooxygenase acuC converts it to m-hydroxybenzyl alcohol, which is further converted to gentisyl alcohol by the cytochrome P450 monooxygenase acuD (Probable). Gentisyl alcohol is further oxidized by the oxidoreductase acuE that probably catalyzes hydroxylation of the aromatic ring (Probable). The aromatic system might then be opened by oxidation through a Baeyer-Villiger type of oxidation, which could be catalyzed by acuF, with the carboxylic acid at C-1 subsequently reduced to an aldehyde by acuG (Probable). Subsequently, a hemiacetal is formed, before the dehydrogenase acuH would reduce the double bond between C-4 and C-6 (Probable). Finally, keto-enol tautomerism results in formation of aculinic acid, which exists as two diastereomers (both R/S configurations at C-1) by non-enzymatic hemiacetal formation (Probable). The carboxypeptidase acuI could be involved in the linking of aculinic acid to an aculene A moiety produced by the aculene biosynthesis cluster and which leads to the production of aculin A (Probable). AcuI may also be involved in the attachment of proline to aculinic acid to form epi-aculins A and B (Probable)

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site447Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase acuC
  • EC number
  • Alternative names
    • Aculin biosynthesis cluster protein C
    • m-cresol hydrolase acuC

Gene names

    • Name
      acuC
    • ORF names
      ASPACDRAFT_45673

Organism names

Accessions

  • Primary accession
    A0A1L9WN31

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane3-23Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004504151-531Cytochrome P450 monooxygenase acuC

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    531
  • Mass (Da)
    60,559
  • Last updated
    2017-03-15 v1
  • Checksum
    8B45EF19F01AF12B
MQPIVWLLGGAIALLVVVIRAAWTYGHRNQDMPSGPPTLPFIGNAHLIPKSYTHIQFTAWARQYGGLYMLKVGNSNMAVVTDRRIVKEVLDSKSSLYSHRPHSFVSHELITQGDHLLVMHYGPKWRTFRRLVHQHLMESMVDSQHLPIVNAEAIQLVRDYMLDPEHHMAHPKRFSNSITNSIVFGIRTADRHGSNMNRLYTLMEQWSEIMETGATPPVDIFPWLKRLPEALFGHYVTRARAIGAQMETLYEDILQRVEKRRSGGVHLDTFMDRVIASQDRNQLPRHQLAFIGGVLMEGGSDTSSSLTLAIVQALTLHPEVQRKAHAEIDAVVGHARSPVWDDLARLPYINMIIKEGHRWRPILPLCFPHALGQDDWVDGKFLPKGTMVVVNTWGMHMDPDHRLNQKYDPAKFVPERFAEHPALAPEYVPGAWENRDHYGYGVSRRICPGIHLAERNMFLAIAKLLWAFEFQPGPEGEPCDSDPVTGYQHGFLYCAKPYSTRPVLRSESIRETVEREFALAQREVFSTFTEG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KV878982
EMBL· GenBank· DDBJ
OJJ97582.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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