A0A1F9AM76 · A0A1F9AM76_9DELT
- ProteinThiamine pyrimidine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids336 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
Catalytic activity
- N6-(pyridoxal phosphate)-L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + L-histidyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 2 Fe3+ + 4 H2O = L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + (2S)-2-amino-5-hydroxy-4-oxopentanoyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 3-oxopropanoate + 2 Fe2+ + 2 H+This reaction proceeds in the forward direction.
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Biological Process | thiamine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameThiamine pyrimidine synthase
Gene names
Organism names
- Taxonomic lineageBacteria > Deltaproteobacteria
Accessions
- Primary accessionA0A1F9AM76
Proteomes
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 47-257 | SsuA/THI5-like | ||||
Sequence: ASVLGDIYADVNGFFARQGLEVDVKAGGPERDAIMELELGRTEFGVASADQVIRALSKGARLKVIAQIFQVNPLQWMYRPDQMRIETLADLKGRRLGVTFGGNDESILRTLLTKGGLGENDVELFSVRNDFTPFLTKKVNFWPVYRNTQAVFLEAKLKDAGEPVEYFDPAAYGVKFVANSVITSMDIFENRPQLVRRFIKGLMEGWKAALD |
Sequence similarities
Belongs to the NMT1/THI5 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length336
- Mass (Da)37,970
- Last updated2017-02-15 v1
- Checksum51767CCEFC896050