A0A1F0UCR0 · A0A1F0UCR0_9PAST

Function

function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site12-13NAD+ (UniProtKB | ChEBI)
Binding site34NAD+ (UniProtKB | ChEBI)
Binding site78NAD+ (UniProtKB | ChEBI)
Binding site120NAD+ (UniProtKB | ChEBI)
Binding site149-151D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Active site150Nucleophile
Site177Activates thiol group during catalysis
Binding site180D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site209-210D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site232D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site319NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Biological Processglucose metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glyceraldehyde-3-phosphate dehydrogenase
  • EC number

Gene names

    • ORF names
      HMPREF2948_01425

Organism names

  • Taxonomic identifier
  • Strain
    • HMSC073C03
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Haemophilus

Accessions

  • Primary accession
    A0A1F0UCR0

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain3-150Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding
Region186-205Disordered

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    339
  • Mass (Da)
    36,114
  • Last updated
    2017-02-15 v1
  • Checksum
    CC8060C63874F908
MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEVKDGNLVVNGKTIRVTSERDPANLNWGAIGVDVAVEATGLFLTDETARKHITAGAKKVVLTGPSKDSTPMFVRGVNFGAYAGQDIVSNASCTTNCLAPLARVVHETFGIKDGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTPNVSVVDLTVNLEKPATYEQIKQAIKDAAEGKTFNGELKGVLGYTEDAVVSTDFNGCTLTSVFDADAGIALTDSFVKLVSWYDNETGYSNKVLDLVAHVYNYKG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LTVN01000047
EMBL· GenBank· DDBJ
OFQ17621.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp