A0A1D8EJF9 · IF4E1_SOLPI
- ProteinEukaryotic translation initiation factor 4E-1
- GeneeIF4E1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids231 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (By similarity).
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (By similarity).
Key component of recessive resistance to potyviruses (PubMed:27655175).
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (By similarity).
Key component of recessive resistance to potyviruses (PubMed:27655175).
(Microbial infection) Susceptibility host factor required for viral infection (e.g. potato virus Y (PVY) and tobacco etch virus (TEV)) by recruiting viral RNAs to the host ribosomal complex via an interaction with viral genome-linked protein (VPg).
Miscellaneous
Displayed sequence confers sensistivity to potyviruses (e.g. potato virus Y (PVY) and tobacco etch virus (TEV)).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 74-79 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: RQTAWG | ||||||
Binding site | 106 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 124-125 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: WE | ||||||
Binding site | 174-179 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: RAKGEK | ||||||
Binding site | 219-223 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: KRLDR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | RNA binding | |
Molecular Function | translation initiation factor activity | |
Biological Process | defense response to virus | |
Biological Process | translational initiation |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameEukaryotic translation initiation factor 4E-1
- Short nameseIF4E-1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon
Accessions
- Primary accessionA0A1D8EJF9
- Secondary accessions
Phenotypes & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 54 | in strain: LA0411, LA1420 and LA0409, allele pot12, haplotypes 1 and 4 | ||||
Sequence: V → K | ||||||
Natural variant | 58 | in strain: LA1478, LA1582 and LA1593, haplotype 2 | ||||
Sequence: L → V | ||||||
Natural variant | 112 | in strain: LA0411, allele pot12, haplotype 4 | ||||
Sequence: D → G | ||||||
Natural variant | 151 | in strain: LA1245, haplotype 3 | ||||
Sequence: T → M |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454062 | 1-231 | Eukaryotic translation initiation factor 4E-1 | |||
Sequence: MAAAEMERTMSFDAAEKLKAADGGGGEVDDELEEGEIVEESNDTASYLGKEITVKHPLEHSWTFWFDNPTTKSRQTAWGSSLRNVYTFSTVEDFWGAYNNIHHPSKLIMGADFHCFKHKIEPKWEDPVCANGGTWKMSFSKGKSDTSWLYTLLAMIGHQFDHGDEICGAVVSVRAKGEKIALWTKNAANETAQVSIGKQWKQFLDYSDSVGFIFHDDAKRLDRNAKNRYTV | ||||||
Disulfide bond | 129↔167 | |||||
Sequence: CANGGTWKMSFSKGKSDTSWLYTLLAMIGHQFDHGDEIC |
Post-translational modification
According to the redox status, the Cys-129-Cys-167 disulfide bridge may have a role in regulating protein function by affecting its ability to bind capped mRNA.
Keywords
- PTM
Interaction
Subunit
EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.
(Microbial infection) Interacts with potyvirus viral genome-linked protein (VPg); this interaction is possible in susceptible hosts but impaired in resistant plants.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 56-59 | EIF4G-binding | ||||
Sequence: HPLE | ||||||
Region | 66-102 | EIF4G-binding | ||||
Sequence: FDNPTTKSRQTAWGSSLRNVYTFSTVEDFWGAYNNIH | ||||||
Region | 150-159 | EIF4G-binding | ||||
Sequence: YTLLAMIGHQ |
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length231
- Mass (Da)26,042
- Last updated2022-02-23 v2
- Checksum0DBFA3141790555C
Polymorphism
Variant present in the strains cv. LA0411, cv. LA1420 and cv. LA0409, allele pot12, haplotypes 1 and 4, is associated with an increased resistance to potato virus Y (PVY) isolates LYE90 and N605 but not to PVY isolate SON41g and to tobacco etch virus (TEV) isolates CAA10.