A0A1B0UVC5 · A0A1B0UVC5_9NEOB

  • Protein
    V(D)J recombination-activating protein 1
  • Gene
    Rag-1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.

GO annotations

AspectTerm
Cellular ComponentDNA recombinase complex
Cellular Componentendodeoxyribonuclease complex
Cellular Componentnucleus
Molecular Functiondouble-stranded DNA endonuclease activity
Molecular Functionhistone binding
Molecular Functionprotein homodimerization activity
Molecular Functionsequence-specific DNA binding
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological Processadaptive immune response
Biological Processchromatin organization
Biological Processpre-B cell allelic exclusion
Biological ProcessT cell differentiation in thymus
Biological ProcessV(D)J recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    V(D)J recombination-activating protein 1
  • EC number

Gene names

    • Name
      Rag-1
    • Synonyms
      RAG1

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Neobatrachia > Hyloidea > Brachycephalidae > Ischnocnema

Accessions

  • Primary accession
    A0A1B0UVC5

Subcellular Location

Keywords

  • Cellular component

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the RAG1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    182
  • Mass (Da)
    20,175
  • Last updated
    2016-11-02 v1
  • Checksum
    1AC4855356F2BC34
LNRSVDEYPVDTISKRFRYDAALVSTLKDMEEDILEGLKSKDLEEYLSGPFTVVIKESCDGMGDVSEKHGCGPPVPEKAVRFSFTIMTISVPNSNNGSVRVFEEAKPNSELCCKPVCLMLADESDHETLTAILGPLIAERECMKSSELLLEIGGILRTFKFIFRGTGYDEKLVREVEGLEAS

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue182

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KT590226
EMBL· GenBank· DDBJ
AMK38550.1
EMBL· GenBank· DDBJ
Genomic DNA
KT590227
EMBL· GenBank· DDBJ
AMK38551.1
EMBL· GenBank· DDBJ
Genomic DNA
KT590229
EMBL· GenBank· DDBJ
AMK38553.1
EMBL· GenBank· DDBJ
Genomic DNA
KT590230
EMBL· GenBank· DDBJ
AMK38554.1
EMBL· GenBank· DDBJ
Genomic DNA
KT590232
EMBL· GenBank· DDBJ
AMK38556.1
EMBL· GenBank· DDBJ
Genomic DNA
KY781302
EMBL· GenBank· DDBJ
ARJ32429.1
EMBL· GenBank· DDBJ
Genomic DNA
KY781315
EMBL· GenBank· DDBJ
ARJ32442.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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