A0A154QXK9 · A0A154QXK9_9GAMM
- ProteintRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- GenemiaB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids463 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.
Catalytic activity
- [sulfur carrier]-SH + AH2 + N6-dimethylallyladenosine37 in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteine
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 17 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 54 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 88 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 162 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 166 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 169 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | methylthiotransferase activity | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Rhodanobacteraceae > Rhodanobacter
Accessions
- Primary accessionA0A154QXK9
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-125 | MTTase N-terminal | ||||
Sequence: HKLFIKTHGCQMNEYDSAKMADVLKASHGLELTQDESEADVILINTCSIREKAQEKVFSQLGRWKQHKQGGKPVLIGVGGCVASQEGAAIVKRAPYVDLVFGPQTLHRLPELIEKQRE | ||||||
Domain | 148-391 | Radical SAM core | ||||
Sequence: RAEGPTAFVSIMEGCSKYCSYCVVPYTRGEEISRPFDDVLVEVAQLAAQGVREVNLLGQNVNAYRGPTHDDADSHLGASRDGGYADLAVLIHAIAQIDGIGRIRFTTSHPLEFSDSLIEAYANVPQLANYLHLPVQAGSDRILGAMKRGYTALEFKQKIRKLRAVRPDICVSSDFIVGFPGETDEDFEKTMKLIDDVGFDQSFSFIFSARPGTPAANLADDTPMAVKHQRLERLQAAINANARK | ||||||
Domain | 394-457 | TRAM | ||||
Sequence: QAMVGTVQRVLVEKPSARNPDELSGRTENMRYVNFAGHPRLIGRFVDVVITDAMSNSLRGRVRL |
Sequence similarities
Belongs to the methylthiotransferase family. MiaB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length463
- Mass (Da)51,015
- Last updated2016-06-08 v1
- Checksum7F2E2304919723CB