A0A0U5GGX2 · AUST_ASPCI

  • Protein
    Short chain dehydrogenase ausT
  • Gene
    ausT
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

Short chain dehydrogenase; part of the gene cluster that mediates the biosynthesis of calidodehydroaustin, a fungal meroterpenoid (PubMed:28233494, PubMed:29076725).
The first step of the pathway is the synthesis of 3,5-dimethylorsellinic acid by the polyketide synthase ausA (PubMed:28233494).
3,5-dimethylorsellinic acid is then prenylated by the polyprenyl transferase ausN (PubMed:28233494).
Further epoxidation by the FAD-dependent monooxygenase ausM and cyclization by the probable terpene cyclase ausL lead to the formation of protoaustinoid A (By similarity).
Protoaustinoid A is then oxidized to spiro-lactone preaustinoid A3 by the combined action of the FAD-binding monooxygenases ausB and ausC, and the dioxygenase ausE (By similarity).
Acid-catalyzed keto-rearrangement and ring contraction of the tetraketide portion of preaustinoid A3 by ausJ lead to the formation of preaustinoid A4 (By similarity).
The aldo-keto reductase ausK, with the help of ausH, is involved in the next step by transforming preaustinoid A4 into isoaustinone which is in turn hydroxylated by the P450 monooxygenase ausI to form austinolide (By similarity).
The cytochrome P450 monooxygenase ausG modifies austinolide to austinol (By similarity).
Austinol is further acetylated to austin by the O-acetyltransferase ausP, which spontaneously changes to dehydroaustin (PubMed:28233494).
The cytochrome P450 monooxygenase ausR then converts dehydroaustin is into 7-dehydrodehydroaustin (PubMed:28233494).
The hydroxylation catalyzed by ausR permits the O-acetyltransferase ausQ to add an additional acetyl group to the molecule, leading to the formation of acetoxydehydroaustin (PubMed:28233494).
The short chain dehydrogenase ausT catalyzes the reduction of the double bond present between carbon atoms 1 and 2 to convert 7-dehydrodehydroaustin into 1,2-dihydro-7-hydroxydehydroaustin (PubMed:28233494).
AusQ catalyzes not only an acetylation reaction but also the addition of the PKS ausV diketide product to 1,2-dihydro-7-hydroxydehydroaustin, forming precalidodehydroaustin (PubMed:28233494).
Finally, the iron/alpha-ketoglutarate-dependent dioxygenase converts precalidodehydroaustin into calidodehydroaustin (PubMed:28233494).

Miscellaneous

In A.calidoustus, the austinoid gene cluster lies on a contiguous DNA region, while clusters from E.nidulans and P.brasilianum are split in their respective genomes. Genetic rearrangements provoked variability among the clusters and E.nidulans produces the least number of austionoid derivatives with the end products austinol and dehydroaustinol, while P.brasilianum can produce until acetoxydehydroaustin, and A.calidoustus produces the highest number of identified derivatives.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site105NADP+ (UniProtKB | ChEBI)
Binding site137NADP+ (UniProtKB | ChEBI)
Active site249Proton donor
Binding site249NADP+ (UniProtKB | ChEBI)
Binding site253NADP+ (UniProtKB | ChEBI)
Active site263Proton donor

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Short chain dehydrogenase ausT
  • EC number
  • Alternative names
    • Austinoid biosynthesis cluster protein T

Gene names

    • Name
      ausT
    • ORF names
      ASPCAL14362

Organism names

  • Taxonomic identifier
  • Strain
    • SF006504
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes

Accessions

  • Primary accession
    A0A0U5GGX2

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004538711-411Short chain dehydrogenase ausT

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    411
  • Mass (Da)
    46,849
  • Last updated
    2016-03-16 v1
  • Checksum
    0FCED3863BE12CB4
MVDKSNKVALVLGATGISGWALAKNALSYPSRSTFQRVIGLMHRPRTVEETGLPNDPRLELYSGVDLQGDLNHVVVKLKEKIPRIEEVTHVYYLAYMTLQTCSGDMRQFRDANVAMTYTAVHACDRLCPNMEFFVLQTGTNAYGVACFDYLRQTQLQVPLRESNPRVPRPWSDTLFYYAQADLIKEANKGKAWKWCEVRPDQIVGHTPAQVSVPYVAPMALYLALYRYINGPGARVQFPGTVRNYHHTYTDVRPDTMARAELYLSLVKSDESHGEAFNIADTDTPGPWSAKWPSICRYFGLEASETIDDEWTDIDGWWYANQTAYERMCAEYGLQKQVMSPTAWSLMKMGHTLIDQNRELCLDKIRGLGFTEDHPVGSSYFQVFEDFERMKIIPPKVVLASPRCRDVLHQC

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CDMC01000024
EMBL· GenBank· DDBJ
CEL11259.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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