A0A0N0TTV2 · A0A0N0TTV2_9ACTN
- Proteinassimilatory sulfite reductase (ferredoxin)
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids846 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reduction of sulfite to sulfide, a step in the biosynthesis of sulfur-containing amino acids and cofactors.
Catalytic activity
- 3 H2O + hydrogen sulfide + 6 oxidized [2Fe-2S]-[ferredoxin] = 7 H+ + 6 reduced [2Fe-2S]-[ferredoxin] + sulfite
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 [4Fe-4S] cluster per subunit.
Note: Binds 1 siroheme per subunit.
Pathway
Nitrogen metabolism; nitrate reduction (assimilation).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 642 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 648 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 682 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 686 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 686 | Fe (UniProtKB | ChEBI) of siroheme (UniProtKB | ChEBI); axial binding residue | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | heme binding | |
Molecular Function | metal ion binding | |
Molecular Function | NADP binding | |
Molecular Function | sulfite reductase (ferredoxin) activity | |
Biological Process | nitrate assimilation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameassimilatory sulfite reductase (ferredoxin)
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionA0A0N0TTV2
Proteomes
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 13-302 | FAD/NAD(P)-binding | ||||
Sequence: TIVLVGHGMVGQRFLEALADRGVTGRARIVVLCEEPRPAYDRVQLTSYFSGKTPDDLSVVGAGFMAEHGIELYVGDAAASVDREARTVTARSGRVFAYDTLVLATGSYPFVPPVPGKDARGCFVYRTIEDLLAIEEYAKTATTGAVVGGGLLGLEAAGALKGLGLETHVVEFAPRLMPVQVDEGGGAALLRTIERMGLGVHTGTGTQEVVTADGAVTGMRLSDGSELAVDMVVFSAGVRPRDQLARDCGLAVGERGGIAVDELCRTSDPAVFAIGECALAVDGRVYGLVA | ||||||
Domain | 332-390 | NADH-rubredoxin oxidoreductase C-terminal | ||||
Sequence: STKLKLLGVDVASFGDAHGTAEGCLDVVYADSRSGVYKKLVVDREGVLLGGVLVGDADS | ||||||
Domain | 431-473 | BFD-like [2Fe-2S]-binding | ||||
Sequence: VICSCHNVTKHAIGQCSSLAEVKKCTKAGTGCGSCVKVIEKLL | ||||||
Domain | 561-622 | Nitrite/Sulfite reductase ferredoxin-like | ||||
Sequence: QRNGSYSVVPRIPGGEITPDKLIVIGEVARDYGLYTKITGGQRIDLFGARVDQLPAIWTRLV | ||||||
Domain | 633-769 | Nitrite/sulphite reductase 4Fe-4S | ||||
Sequence: GKSLRTVKSCVGQTWCRYGVQDSVKMAIHLELRYRGLRAPHKLKSAVSGCARECAEAQSKDFGIIATANGWNLYVGGNGGATPRHADLLAQDLSDAELIRLIDRFLMFYIRTADRLERTSTWLERLEGGLEHLKDVV |
Sequence similarities
Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length846
- Mass (Da)89,721
- Last updated2015-12-09 v1
- Checksum3FD3DCC46D96D2C0
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LGEE01000115 EMBL· GenBank· DDBJ | KOX30841.1 EMBL· GenBank· DDBJ | Genomic DNA |