A0A0G9LIH2 · A0A0G9LIH2_9CLOT

  • Protein
    Queuine tRNA-ribosyltransferase
  • Gene
    tgt
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site93Proton acceptor
Binding site93-97substrate
Binding site147substrate
Binding site198substrate
Binding site225substrate
Active site275Nucleophile
Binding site313Zn2+ (UniProtKB | ChEBI)
Binding site315Zn2+ (UniProtKB | ChEBI)
Binding site318Zn2+ (UniProtKB | ChEBI)
Binding site344Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • ORF names
      AAT22_07945

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • C8
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium

Accessions

  • Primary accession
    A0A0G9LIH2

Proteomes

Interaction

Subunit

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain13-376tRNA-guanine15 transglycosylase-like
Region256-262RNA binding
Region280-284RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    380
  • Mass (Da)
    43,182
  • Last updated
    2015-09-16 v1
  • Checksum
    F8813298E2E2FBCC
MSKKRYTLLKKDGEARRGRFETPHGTIETPVFMNVGTLAVIKGAVSTMDLKEIGCQVELSNTYHLHLRPSDKVVASLGGLHNFMNWDRPILTDSGGFQVFSLSGMRKIKEEGVYFSSHIDGKKIFMGPEESMQIQSNLASTIAMAFDECIPNPSTRDYVEKSVARTTRWLERCKKEMDRLNSLPETINKEQMLFGINQGGCYEDIRIEHAKTITKMDLDGYAIGGLAVGETHEEMYRIIDAVVPYLPEDKPIYLMGVGTPSNILEAVDRGVDFFDCVLPARNGRHGHVFTKEGKINLMNAKFELDTRPIDEGCQCPACRSYTRAYIRHLFKAKEMLAMRLCVLHNLYFYNKLMEDIRSAIDGGYFKEFKDEKLKEWNGKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LBMX01000049
EMBL· GenBank· DDBJ
KLE16108.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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