A0A0D6P949 · A0A0D6P949_9PROT

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site58[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site87(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site87dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site87isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site120(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site120dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site120isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site142[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site174(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site174dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site174isopentenyl diphosphate (UniProtKB | ChEBI)
Active site176Proton donor
Binding site215(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site245[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site273(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site273dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site273isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site274(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site274dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site274isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site275(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site275dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site275isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site318(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site318dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site318isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      Asru_0497_02

Organism names

Accessions

  • Primary accession
    A0A0D6P949

Proteomes

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-15Polar residues
Region1-39Disordered

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    358
  • Mass (Da)
    38,119
  • Last updated
    2015-05-27 v1
  • Checksum
    A6902ABFE58C54C6
MTTDGRPSIEHSACQTGTVAPGRAGIEPPPGAGNAPAPGGATTVAPLRVLLAGPRGFCAGVDRAIRVVEEALRQYGAPVYVRHEIVHNRTVVETLERQGAVFVEDLTEVPEDARVVFSAHGVPKSVPAEARRRHLLYLDATCPLVSKVHREAERHFAGGGAEARHILMIGHAGHPEVEGTMGQLPPGAMTLVQDADEARSVAPPDPTRLAFITQTTLSVDDTAEIVTILRDRFPHIVGPRHEDICYATTNRQAAVKAIAPDCDLVIVIGSPNSSNSQRLREVAERAGAARAVLLPDASGLDWRWLDGVGTLGLSAGASAPEVLVDALIDRLRGRYALTIEERRVTTEDVVFKLPAPLG

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-15Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BANB01000497
EMBL· GenBank· DDBJ
GAN77886.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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