A0A0C3N1R1 · A0A0C3N1R1_9RHOO

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site15[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site44substrate
Binding site77substrate
Binding site99[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site127substrate
Active site129Proton donor
Binding site167substrate
Binding site197[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site225-227substrate
Binding site269substrate

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      PO78_1466

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SWB20
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Rhodocyclales > Zoogloeaceae > Thauera

Accessions

  • Primary accession
    A0A0C3N1R1

Proteomes

Family & Domains

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    312
  • Mass (Da)
    33,888
  • Last updated
    2015-04-01 v1
  • Checksum
    9ABF26A1A8226307
MNDKEILLANPRGFCAGVERAIEIVERALKRYGAPIYVRHEVVHNKFVVDDLRAKGAIFVDELDEVPTGNTVIFSAHGVSLAVREEAERRGLRVFDATCPLVTKVHVEVARMRDQGKEIVMIGHKGHPEVEGTMGQVDGGIHLVETPADVAGLQVADPEQLAYVTQTTLSVDDAAAIVDALRTRFPRVVGPKKDDICYATQNRQDAVKFMTPYADVVFVVGSKNSSNSNRLREVAALRGVPAYLIDNAAGIDPAWIEGKRRIGVTAGASAPEVLVAEVIARLEELSGGAVRNLDGVPERVTFALPKELQDPR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JTDM01000020
EMBL· GenBank· DDBJ
KIN89643.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp