A0A097KPK6 · A0A097KPK6_9VIRI
- ProteinDNA-directed RNA polymerase subunit alpha
- GenerpoA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids412 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | membrane | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit alpha
- EC number
- Short namesPEP
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Klebsormidiophyceae > Klebsormidiales > Klebsormidiaceae > Interfilum
Accessions
- Primary accessionA0A097KPK6
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 364-384 | Helical | ||||
Sequence: IYNLLCIFIFYFFQFFFYYLL |
Keywords
- Cellular component
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-263 | Alpha N-terminal domain (alpha-NTD) | ||||
Sequence: MENFLLTCIESRVENNTSFYGRFQLGPFEGGQGLTIANAFRRVLLSELSGLAIVGVEIQGVTHEYSYLKGIRESILDILLNLKKIVLTSDFDCKEKQIAYLNVQGPAKIKAKDLKLPLSLQCVDPEQYIATLSHDGNLVMKLLICHGKNYSIQNNEYKNLTKKISFFEKNKIEKNLFKNLLPIDSIYMPIKKVNYIVESDDDTIELKDRIILEIWTNGSIHPRQAIHNAAKILVQLFFPFQETQPLKSLFLNTKNIKVLNYLE | ||||||
Domain | 20-243 | DNA-directed RNA polymerase RpoA/D/Rpb3-type | ||||
Sequence: YGRFQLGPFEGGQGLTIANAFRRVLLSELSGLAIVGVEIQGVTHEYSYLKGIRESILDILLNLKKIVLTSDFDCKEKQIAYLNVQGPAKIKAKDLKLPLSLQCVDPEQYIATLSHDGNLVMKLLICHGKNYSIQNNEYKNLTKKISFFEKNKIEKNLFKNLLPIDSIYMPIKKVNYIVESDDDTIELKDRIILEIWTNGSIHPRQAIHNAAKILVQLFFPFQET | ||||||
Region | 279-412 | Alpha C-terminal domain (alpha-CTD) | ||||
Sequence: IDKKLGSLDIGNLDLSLRPYTCLKRANIHTIGDLLKYSKEELLLLKNFGKRSLEEVETNLFQIGLKLKNTNGIDITLIKNKLKKQIYNLLCIFIFYFFQFFFYYLLVENISKKKKNVFTAAQRMKSSVASPAKQ |
Domain
The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.
Sequence similarities
Belongs to the RNA polymerase alpha chain family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length412
- Mass (Da)47,400
- Last updated2015-01-07 v1
- Checksum592E51A291A231A4
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KM462881 EMBL· GenBank· DDBJ | AIT95124.1 EMBL· GenBank· DDBJ | Genomic DNA |