A0A095AMW7 · MLES_LEUME

Function

function

Involved in the malolactic fermentation (MLF) of wine, which results in a natural decrease in acidity and favorable changes in wine flavors. Catalyzes the decarboxylation of L-malate to L-lactate.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

NAD+ (UniProtKB | Rhea| CHEBI:57540 )

Activity regulation

Oxamate, fructose-1,6-diphosphate and L-lactate act as non-competitive inhibitors, whereas succinate, citrate and tartrate isomers produce a competitive inhibition.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.043 mMNAD
16.7 mM(S)-malate

pH Dependence

Optimum pH is 4.35.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site92Proton donor
Active site165Proton acceptor
Binding site165substrate
Binding site236Mn2+ (UniProtKB | ChEBI)
Binding site237Mn2+ (UniProtKB | ChEBI)
Binding site260Mn2+ (UniProtKB | ChEBI)
Binding site293-296NAD+ (UniProtKB | ChEBI)
Binding site405NAD+ (UniProtKB | ChEBI)
Binding site450NAD+ (UniProtKB | ChEBI)
Binding site450substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functioncarboxy-lyase activity
Molecular Functionmalic enzyme activity
Molecular Functionmalolactic enzyme activity
Molecular Functionmanganese ion binding
Molecular FunctionNAD binding
Molecular Functionoxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Biological Processmalate metabolic process
Biological Processmalolactic fermentation
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Malolactic enzyme
  • EC number
  • Short names
    MLE

Gene names

    • Name
      mleS
    • ORF names
      LH61_04880

Organism names

Accessions

  • Primary accession
    A0A095AMW7

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004356721-542Malolactic enzyme

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the malic enzymes family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    542
  • Mass (Da)
    59,205
  • Last updated
    2014-11-26 v1
  • Checksum
    F2B6A27F7E2AA86E
MNTTGYDILRNPFLNKGTAFSEAERQQLGLTGTLPSQIQTIEEQAEQAYKQFQAKSPLLEKRIFLMNLFNENVTLFYHLMDQHVSEFMPIVYDPVVAESIEQYNEIYTNPQNAAFLSVDRPEDVENALKNAAAGRDIKLVVVTDAEGILGMGDWGVNGVDIAVGKLMVYTAAAGIDPATVLPVSIDAGTNNKELLHNPLYLGNKHERIAGEQYLEFIDKFVTAEQNLFPESLLHWEDFGRSNAQVILDKYKESIATFNDDIQGTGMIVLAGIFGALNISKQKLVDQKFVTFGAGTAGMGIVNQIFSELKQAGLSDDEARNHFYLVDKQGLLFDDTEGLTAAQKPFTRSRKEFVNPEQLINLETIVKELHPTVLIGTSTQPGTFTETIVKSMAENTERPIIFPLSNPTKLAEATAEDLIKWTGGKALVATGIPAADVDYKGVTYKIGQGNNALIYPGLGFGLVASTAKLLTQETISAAIHALGGLVDTDEPGAAVLPPVSNLTDFSQKIAEITAQSVVNQGLNREKIVDPKQAVQDAKWSAEY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JRGZ01000002
EMBL· GenBank· DDBJ
KGB50834.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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