A0KFC0 · MURQ_AERHH
- ProteinN-acetylmuramic acid 6-phosphate etherase
- GenemurQ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids298 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activity
- N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + (R)-lactate
Pathway
Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.
Amino-sugar metabolism; N-acetylmuramate degradation.
Cell wall biogenesis; peptidoglycan recycling.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 85 | Proton donor | |||
Active site | 116 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbohydrate derivative binding | |
Molecular Function | carbon-oxygen lyase activity | |
Molecular Function | ether hydrolase activity | |
Biological Process | 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process | |
Biological Process | amino sugar catabolic process | |
Biological Process | N-acetylmuramic acid catabolic process | |
Biological Process | peptidoglycan turnover |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylmuramic acid 6-phosphate etherase
- EC number
- Short namesMurNAc-6-P etherase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Aeromonadales > Aeromonadaceae > Aeromonas
Accessions
- Primary accessionA0KFC0
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000009108 | 1-298 | N-acetylmuramic acid 6-phosphate etherase | ||
Interaction
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length298
- Mass (Da)31,118
- Last updated2006-12-12 v1
- MD5 ChecksumDD1DA9340B479897265D95F547433BFF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000462 EMBL· GenBank· DDBJ | ABK36652.1 EMBL· GenBank· DDBJ | Genomic DNA |