A0AMA3 · GSHAB_LISW6

Function

function

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 magnesium or manganese ions per subunit.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site548-606ATP (UniProtKB | ChEBI)
Binding site728Mg2+ 1 (UniProtKB | ChEBI)
Binding site728Mn2+ 1 (UniProtKB | ChEBI)
Binding site745Mg2+ 1 (UniProtKB | ChEBI)
Binding site745Mg2+ 2 (UniProtKB | ChEBI)
Binding site745Mn2+ 1 (UniProtKB | ChEBI)
Binding site745Mn2+ 2 (UniProtKB | ChEBI)
Binding site747Mg2+ 2 (UniProtKB | ChEBI)
Binding site747Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionglutamate-cysteine ligase activity
Molecular Functionglutathione synthase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutathione biosynthesis bifunctional protein GshAB
  • Alternative names
    • Gamma-GCS-GS
      (GCS-GS
      )

Including 2 domains:

  • Recommended name
    Glutamate--cysteine ligase
  • EC number
  • Alternative names
    • Gamma-ECS
      (GCS
      )
    • Gamma-glutamylcysteine synthetase
  • Recommended name
    Glutathione synthetase
  • EC number
  • Alternative names
    • GSH synthetase
      (GS
      ; GSH-S
      ; GSHase
      )
    • Glutathione synthase

Gene names

    • Name
      gshAB
    • Synonyms
      gshF
    • Ordered locus names
      lwe2717

Organism names

Accessions

  • Primary accession
    A0AMA3

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10001337201-776Glutathione biosynthesis bifunctional protein GshAB

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-354Glutamate--cysteine ligase
Domain521-775ATP-grasp

Sequence similarities

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    776
  • Mass (Da)
    88,615
  • Last updated
    2006-11-28 v1
  • Checksum
    9DDA45402F88588D
MIKLDMTILDSLKENKALRKLLFSGHFGLEKENIRVTSDGKLALTPHPAIFGPKEDNPYIKTDFSESQIEMITPVTDSIDDVYNWLENLHNIVSLRSKNELLWPSSNPPILPAEKDIPIAEYKTPDSPDRKYREHLAQGYGKKIQLLSGIHYNFSFPEALIDGLYDEISLPNESKRDFKNRLYLKVAKYFMKNRWLLIYLTGASPVYLADFTKTKQEEKLRDGSSALHDGISLRNSNAGYKNKESLYVDYNSFDAYISSISNYIEAGKIESMREFYNPIRLKNAHTDQTVESLAKHGVEYLEIRSIDLNPLEPNGISKEALHFIHLFLIKGLLSEDRELCENNQQLADENENNIALNGLSKPAIKNCDNEEMALADAGLLELDKMNDFIQSLRPEDTYFQAIIEKQKERLLHPEKTIAAQVKEQSATAGFIEFHLNQAKTYMEETEALAYKLIGAEDMELSTQIIWKDAIARGIKVDVLDRAENFLRFQKGDHVEYVKQASKTSKDNYVSVLMMENKVVTKLVLAENNIRVPFGDSFSDQALALEAFSLFKDKQIVVKPKSTNYGWGISIFKNKFTTEDYQEALNIAFSYDSSVIIEEFIPGDEFRFLVINDKVEAVLKRVPANVTGDGIHTVRELVEEKNMDPLRGTDHLKPLEKIRTGPEETLMLSMQKLSWDSIPKANETIYLRENSNVSTGGDSIDYTAEMDDYFKEIAIRATQVLDAKICGVDIIVPRETIDRDKHAIIELNFNPAMHMHCFPYQGEQKKIGDKILDFLFE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AM263198
EMBL· GenBank· DDBJ
CAK22135.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp